Current Issue : January-March Volume : 2019 Issue Number : 1 Articles : 2 Articles
Three simple, accurate, rapid and sensitive methods (A, B and C) have been developed for the estimation of emtricitabine in its pharmaceutical dosage form. The Method A is based on the formation of orange red colored chromogen, due to reaction of emtricitabine with p-dimethyl amino cinnamaldehyde (PDAC) reagent in the presence of methanol, which exhibits λmax at 538 nm. Method B is based on the reaction of emtricitabine with 3-methyl-2-benzothiazolinone hydrazone (MBTH) in the presence of cerric ammonium sulphate to form a bluish green colored chromogen, which shows maximum absorbance at 635 nm. The Method C is based on the formation of reddish brown colored chromogen with mordant black dye in the presence of Phthalate buffer, which shows absorption maximum at 543 nm. The absorbance-concentration plot is linear over the range of 1-10 mcg/ml for Method A, 1-18 mcg/ml for Method B and 5-90 mcg/ml for Method C. Results of analysis for all the methods were validated statistically and by recovery studies. Literature survey reveals only HPLC methods deals with biological fluids and in fixed pharmaceutical dosage forms. The proposed methods are economical and sensitive for the estimation of emtricitabine in bulk drug and in its formulations....
For estimation of certain properties of molecule, useful information can be obtained from physical and other chemical phenomenon and transformed by mathematical and statistical method which is known as chemometrics. Many calibration problems related to analysis of spectral data can be solved by using chemometrics method. This review article mainly involves different chemometrics method and how we use these chemometrics methods in the optimization of method development. Among these all methods partial least square method has received great attention due to its importance. In recent time, the use of chemometrics is greatly enhanced due to its efficiency in analytical method development. So, the main aim of this review article is to understanding of all chemometrics method and use of those methods in method development....
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